Modern biology has focused on purifying different cells from the body and growing them in culture – often in flat layers on hard plastic dishes that do little to represent the complexity of human biology.
To address this challenge, two branches of 3D cell culture technologies have emerged.
use solid scaffolds, hydrogels and other biomaterials to support individual cells growing in 3D.
use low adhesion plastics, hanging drops and magnetic forces to bring multiple cells in contact with each other.
For making organoids reproducible and available, we need to combine these two techniques.
We developed the Gri3D® platform that combines 3D aggregation with biomaterials to create a 3D culture according to the current gold standard of 2D cell culture.
3D in 2D
With Gri3D® have your complete 3D culture in a single focal plane.
Accuracy & Scalability
With Gri3D® generate hundreds of uniform organoids at once.
Allow survival and growth of even your most sensitive stem cells.
All in One
With Gri3D® use only one platform for your entire organoid protocol.
The Gri3D® platform allows the standardized growth of organoids in large scale.
Success So Far..
Using pluripotent stem cells, we are growing retinal organoids.
These retinal organoids, also called Optic Cups, develop into layered retinal tissues with a ganglion cell layer, an inner nuclear layer and an outer nuclear layer. These Optic Cups express Crx, an early marker for cones and rods, the light-sensing cells of the eye.
Using adult stem cells, we are growing intestinal organoids.
These intestinal organoids, harbor the functional cells of the intestinal epithelium :
Paneth cells producing antimictobial peptides, Goblet cells producing the mucus of the gut, Enteroendocrine cells producing hormones, Enterocytes responsible for nutrient absorption
Using pluripotent stem cells, Gri3D® is used to grow cerebral organoids.
Using fetal stem cells, Gri3D® is used to grow pancreatic organoids.
Using pluripotent stem cells, Gri3D® is used to grow otic organoids.
Organoids can be grown from the stem cells of each person. They open the door to personalized medicine, where we can understand and test the differences from one patient to the next.
For pharmaceutical companies, personalized testing means that drugs that so far failed to show efficacy for the global patient population could be marketed for the specific pool of patients where they do work.
For patients, personalized testing means to pre-select the medications that work for them, resulting altogether in more effective and cost-effective therapies.
With Gri3D®, we can make homogenous populations of organoids
and monitor them separately with high accuracy, in real-time, over time.
Modeling the biology behind cholera infections
Cholera toxin, a toxin produced by Vibrio cholerae bacteria, results in a rapid fluid loss from the intestine, leading to severe dehydration and massive watery diarrhea. This process can be modeled on intestinal organoid cultures, where it results in rapid swelling of the organoids due to hypersecretion of electrolytes and water to the central lumen.